Charlie Kirk, the action group's founder and a political commentator, railed against the flag being hung beside the star-spangled banner and the Texas flag outside the Gaylord Texan Resort in Grapevine, near Dallas, and threatened not to return to the hotel for further events.
He said he had tried to have the flag removed, but had "failed." Conservative activists gathered at the hotel and conference center for Turning Point's Young Women's Leadership Summit over the weekend.
Conservative commentators have hit out at previous shows of solidarity with the LGBTQ+ community this month, and it follows months of calls for boycotts of brands appearing to align with or serve LGBTQ+ customers, including Bud Light and Target. The controversies speak to a wider culture war about the acceptance of LGBTQ+ individuals in public life.